Tracking single quantum dot and its spectrum in free solution with controllable thermal diffusion suppression
Resource
Analytical Biochemistry, 377(2), 176-181
Journal
Analytical Biochemistry
Pages
176-181
Date Issued
2008
Date
2008
Author(s)
Li, Q.
Han, R.
Meng, X. X.
Gai, H. W.
Yeung, E. S.
Abstract
Thermal motions of semiconductor quantum dots (QDs) are suppressed on a dehydrated agarose-modified surface. The diffusion coefficients (D) of particles can be controlled by modifying the surface with an appropriate agarose concentration. The value of D is more than 100 times lower than the theoretical value when the dried agarose surface is made with an 8% agarose solution. This makes it possible to real-time record the diffusion process of single particles and single molecules in low-viscosity solution. A transmission grating installed in front of the charge-coupled device separates the QD fluorescence into the zeroth-order and first-order spectrum. Therefore, the spectrum of dynamic QDs is tracked on the modified surface. Tracking the dynamic QD spectral image is a promising method to explore the process of the molecular interactions in the physiological buffer.
Type
journal article
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