Development of 24 new microsatellite markers in the Crested Serpent Eagle (Spilornis cheela hoya)
Journal
Conservation Genetics Resources
Journal Volume
5
Journal Issue
2
Pages
417-420
Date Issued
2013
Author(s)
Hsu H.-H.
Chang Y.-Y.
Chao M.-C.
Tsao H.-S.
Chan F.-T.
Hsu C.-C.
Abstract
Twenty-four microsatellite markers were developed for the Crested Serpent Eagle (Spilornis cheela hoya) composing a new marker set. The markers were tested on 61 Crested Serpent Eagle individuals. The number alleles ranged from 2 to 8 per locus (average = 3. 8), and the effective number of alleles ranged from 1. 13 to 6. 07 (average = 2. 34). In the new marker set, there were 11 markers with high polymorphism (PIC > 0. 5), and the average HE and HO over all loci was 0. 50 and 0. 49, respectively. The results showed that the new marker set was highly polymorphic as an individual genetic marker compared to the existing cross-species markers previously used for population genetic structure monitoring of the Taiwan Crested Serpent Eagle. In conclusion, the microsatellite marker set developed can be applied as a molecular tool to investigate the genetic structure or phylogeny of the Crested Serpent Eagle. © 2012 Springer Science+Business Media Dordrecht.
SDGs
Type
journal article