神經傳遞物質與實驗性水腦症之關係（於不同年齡之麻醉與自由行走之實驗性水腦動物的研究）
Date Issued
2003-07-31
Date
2003-07-31
Author(s)
郭夢菲
DOI
902314B002430
Abstract
Hydrocephalus is the most common disease in pediatric neurosurgery. It
happens to the old patients, too. The motor and/or behavioral disturbances induced
by hydrocephalus are, in some cases, rapidly reversible by CSF diversioning
procedure suggesting that there may be a functional impairment of neurons.
2
Neonatal hydrocephalus is induced in 3-week old rat by kaolin injection into the
cisterna magna. Striatal extracellular concentrations of the dopamine and its
metabolites in rats, DOPAC and homovanillic acid (HVA), are assayed by
microdialysis and HPLC, 1 and 4 weeks after induction of hydrocephalus (acute and
chronic hydrocephalus, respectively). The assays are also conducted in the
hydrocephalic rat brains with CSF diversion. The tissue concentrations of dopamine,
DOPAC and HVA will be measured by HPLC in various groups of animal. The data
will be compared with the control and sham-operated groups.
We test the hypothesis that CSF accumulation during hydrocephalus will have
effects from mechanical compression, vascular compromise and metabolic
disturbance on the striatum, which is located adjacent to the ventricular system.
Dopaminergic system, one of the major neurotransmitter systems in the striatum, will
be affected during hydrocephalus. The changes of the dopaminergic system during
acute and chronic hydrocephalus may be reversed differently after the CSF diversion.
After that, the hypothesis that the age of the animal might respond differently to the
effect of hydrocephalus will be tested.
In the first year of this project, we are going to use neonatal rats to study the
relationship between the dopaminergic system and hydrocephalus. In the second
year, freely moving rats are going to be used in stead of anesthetized rats to get a
chronological profile of the dopaminergic system during the development of
hydrocephalus. The changes of the dopaminergic system after CSF diversion will be
studies in the freely moving rats, too. In the final year, the changes of dopaminergic
system after hydrocephalus in aged rat brains will be studied. The results will be
compared with the changes in the immature rat brain, which are shown in the first two
years of this project.
Subjects
Rat
Freely moving
Hydrocephalus
Dopamine
Striatum
Microdialysis
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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