Frequency-Scanning MALDI Linear Ion Trap Mass Spectrometer for Large Biomolecular Ion Detection
Resource
ANALYTICAL CHEMISTRY, 83(21), 8273-8277
Journal
Analytical Chemistry
Pages
8273-8277
Date Issued
2011
Date
2011
Author(s)
Lu, I-Chung
Lin, Jung Lee
Lai, Szu-Hsueh
Chen, Chung-Hsuan
Abstract
This study presents the first report on the development of a matrix-assisted laser desorption ionization (MALDI) linear ion trap mass spectrometer for large biomolecular ion detection by frequency scan. We designed, installed, and tested this radio frequency (RF) scan linear ion trap mass spectrometer and its associated electronics to dramatically extend the mass region to be detected. The RF circuit can be adjusted from 300 to 10 kHz with a set of operation amplifiers. To trap the ions produced by MALDI, a high pressure of helium buffer gas was employed to quench extra kinetic energy of the heavy ions produced by MALDI. The successful detection of the singly charged secretory immunoglobulin A ions indicates that the detectable mass-to-charge ratio (m/z) of this system can reach ~385 000 or beyond.
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Type
journal article
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