Induction and Cultivation of Somatic Embryogenic cells of Celery and Its Bioreactor Operation Strategy(2/2)
Date Issued
2004
Date
2004
Author(s)
黃世佑
DOI
922214E002017
Abstract
Enbryogenesis which resembles a zygotic
embryo of pollinated egg is developed from a
single cell. It is developed via globular, heart
and torpedo embryos and to a maturated
embryo. The embryogenesis frequency (EF)
can be promoted through a medium
optimization, elicitor dosages and controlling
gas composition of the bioreactor. Induction of
embryogenetic cells in large scale bioreactors
is most important for mass production of
plantlets and development of artificial seeds.
The celery (Tall Utah) was adopted as a model
system to investigate the effective methods for
enhancing embryogenic cells. For medium
optimization, several carbon sources were
compared for obtaining the high total amount
of embryo cells in a cultivating system.
Nitrogen source and some specific
components, e.g., yeast extract, salicylic acid
and abscisic acid which related the protective
function of plants affecting the embryogenesis
frequency were optimized, ABA (3浵M)
enhanced the EF 2.74 folds compared to
control medium.
Performances in embryo induction for
three different types of bioreactor were
compared. For agitated bioreactor, low shear
stress and well dispersion of cells within the reactor were the essential issue to be sought.
An impeller like hydrofoil exhibited a mild
shear and well lifting capability. Mist trickling
bioreactor had merits for adhering the cells on
the wire mesh which facilitated the
synchronization of the embryo cells.
Subjects
Embryogenesis
Celery
Embryogenesis Frequency
Aggregate size
distribution
distribution
Bioreactor
Publisher
臺北市：國立臺灣大學化學工程學系暨研究所
Type
report
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