Alleviation of Ion Suppression Effect in Cyclodextrin Electrokinetic Chromatography/ Electrospray Ionization Mass Spectrometry Due to Chiral Selector
Date Issued
2009
Date
2009
Author(s)
Hung, Sih-Hua
Abstract
A method capable to couple high concentration cyclodextrin electrokinetic chromatography with electrospray ionization mass spectrometry (CD/EKC-ESI/MS) detection without suffering ion signal suppression was proposed. The concept of liquid junction/low flow interface was employed in this work. A normal polarity was applied across the connecting capillary; therefore, the negative sulfated β-cyclodectrin (S-β-CD) could be remained in liquid junction reservoir due to the electric field. Running buffer containing 20% acetonitrile was filled in the liquid junction reservoir. Because of acetonitrile, the affinity of the analyte with S-β-CD could be reduced, so that the analyte could migrated toward the MS detector by electroosmotic flow. The inlet of capillary electrophoresis was elevated to avoid ACN from entering the separation column to deteriorate the interaction between S-β-CD and the analyte during the capillary electrophoresis(CE) separation. Because the reversed polarity was applied across the separation column, the S-β-CD zone could be filled in the CE separation column to preserve the separation efficiency. Both good chiral separation efficiency and low ion signal suppression could be achieved by this approach.
Subjects
cyclodextrin
capillary electrophoresis
mass spectrometry
liquid junction/low flow interface
Type
thesis
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