The Neuroprotective Effects of Minocyline on Retinal Ischemia-Reperfusion Injury in the rat
Date Issued
2008
Date
2008
Author(s)
Huang, Pei-Yun
Abstract
Minocycline, a microglial inhibitor, has recently been shown to be neuroprotective in various models of cerebral ischemia and degenerative diseases. The purpose of this study was to investigate the neuroprotective effect of intraperitoneal minocycline against the retinal ischemia-reperfusion (IR) injury in the rat. The retinal IR injury model was induced in Sprague-Dawley rats by infusing normal saline (0.9%) into anterior chamber of eye to creat a higher intraocular pressure (IOP) than blood pressure for 45 min. Minocycline was given intraperitoneally at dosages of 45 mg/kg per day and 90 mg/kg per day in rats of IR injury model. The effects were evaluated by electroretinogram (ERG) 3 days and 7 days after ischemia respectively, and compared with the groups treated with saline and methyprenisolone (30mg/kg, IV). Present results have indicated that ischemia-reperfusion (IR) injury caused retinal damage characterized by a decline in electroretinogram b-wave (loss of 74±2% b-wave, n=5) whereas the a-wave was relatively unaffected. Administration of minocycline in both doses ameliorated the damage of retinal function. The b-wave of ERG dropped down 37±6% in the 90 mg/kg/day group ( n=6) and 43±2% in the 45mg/kg/day group (n=6). In the morphometrical histology, the retinal inner plexiform layer/outer nuclear layer (IPL/ONL) ratio was reduced following IR damage compared with that in the normal control. The IPL/ONL ratio was reduced to 44.1% in the IR-control group, 93.4% and 94.1% in the Mino-45 and Mino-90 group. While the IPL/ONL ratio was preserved to 96.7% in the MP group. According to the results, minocycline and methylprednisolone showed significantly good neuroprotective effects demonstrated in histology, but moderate neuroprotective effects in retinal function of the rats under retinal ischemia-reperfusion injury.
Subjects
retinal ischemia-reperfusion injury
ischemia-reperfusion injury
minocycline
electroretinogram
neuroprotective effect
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R94629032-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):dd86c0ce062d9bed524b71db4d910f02