Single-walled carbon nanotubes can induce pulmonary injury in mouse model
Journal
Nano Letters
Journal Volume
8
Journal Issue
2
Pages
437-445
Date Issued
2008
Author(s)
Abstract
Carbon nanotubes are a nanomaterial that is extensively used in industry. The potential health risk of chronic carbon nanotubes exposure has been raised as of great public concern. In the present study, we have demonstrated that intratracheal instillation of 0.5 mg of single-walled carbon nanotubes (SWCNT) into male ICR mice (8 weeks old) induced alveolar macrophage activation, various chronic inflammatory responses, and severe pulmonary granuloma formation. We then used Affymetrix microarrays to investigate the molecular effects on the macrophages when exposed to SWCNT. A biological pathway analysis, a literature survey, and experimental validation suggest that the uptake of SWCNT into the macrophages is able to activate various transcription factors such as nuclear factor κB (NF-κB) and activator protein 1 (AP-1), and this leads to oxidative stress, the release of proinflammatory cytokines, the recruitment of leukocytes, the induction of protective and antiapoptotic gene expression, and the activation of T cells. The resulting innate and adaptive immune responses may explain the chronic pulmonary inflammation and granuloma formation in vivo caused by SWCNT. ? 2008 American Chemical Society.
Other Subjects
Biological pathway; Granuloma formation; Intratracheal instillation; Molecular effects; Pulmonary injury; Health risks; Macrophages; Microarrays; Pulmonary diseases; Transcription factors; Single-walled carbon nanotubes (SWCN); carbon nanotube; cytokine; animal; article; cell culture; chemically induced disorder; disease model; drug effect; immunology; Institute for Cancer Research mouse; lung; lung disease; macrophage; male; mouse; pathology; ultrastructure; Animals; Cells, Cultured; Cytokines; Disease Models, Animal; Lung; Lung Diseases; Macrophages; Male; Mice; Mice, Inbred ICR; Nanotubes, Carbon
Type
journal article