Effects of Black Bean, Astragalus membranaceus, Agaricus blazei and Fermentation Products of Agaricus blazei-leguminous Plants on Serum Lipids of Hamsters
Date Issued
2005
Date
2005
Author(s)
Yang, Ya-Lan
DOI
zh-TW
Abstract
Cardiovascular disease is the main cause of death in the world and it is related to atherosclerosis. It was suggested that high blood lipid and oxidative stress would result in LDL oxidation leading to atherogenesis. Therefore, preventing or delaying the onset of atherosclerosis could start from lowering blood lipid and prolonging the lag phase of LDL oxidation. Recent studies were focused on improving blood lipid and replenishing exogenous antioxidants to delay the progression of atherosclerosis.
Scientific literatures reported that Agaricus blazei, black bean and Astragalus membranaceus showed antioxidative or hypolipidemic effects. The health protective effect of A. blazei was different from black bean and A. membranaceus, but they could complement one another. To use black bean or A. membranaceus as a part of the fermentation medium for the liquid fermentation of A. blazei, the active antioxidative compounds might be transformed into the more bioavailable form with higher biological activity.
Hamster was chosen as the animal model of this study to investigate the effects towards blood lipid and LDL oxidation of the fruiting body of A. blazei, black bean, A. membranaceus and the fermentation products of A. blazei-leguminous. Three animal experiments were conducted to examine the antiatherogenic effects. Animal study I was conducted for 4 weeks and 8 weeks, and the test materials were A. blazei fruiting bodies, black bean and A. membranaceus. Animal study II was conducted to correct the shortcomings of animal study I to proceed the experiment once again. Animal study III was conducted to examine the effects of the fermentation products of A. blazei-leguminous.
The results of animal study I showed that after feeding hamsters 4 weeks with 10% black bean, the hamsters had lower HDL-C in serum, higher hepatic cholesterol and their liver weight and relative fat pad weight increased. Adding 10% A. membranaceus in the test diet could decrease LDL-C in serum of hamsters after 4 weeks. Hamsters fed 8 weeks with 10% black bean and A. membranaceus had higher hepatic cholesterol. Adding 10% black bean or 10% A. blazei in the test diet increased their relative liver weight.
The results of animal study II showed that hamsters fed with 10% black bean or 10% A. membranaceus had higher serum HDL-C and hepatic cholesterol. A. membranaceus added at 10% level in the diet could also reduce thiobarbituric acid reactive substances (TBARS) in the liver. Hamsters fed with 10% A. blazei had lower triglyceride and VLDL-C in serum. Hamsters in Agaricus blazei fruiting bodies, black bean and A. membranaceus groups showed lower levels of serum MDA than high fat high cholesterol group.
The results of animal study III showed that hamsters fed with 10% fermentation product of A. blazei (GF4), which used soybean as a part of fermentation medium, had lower levels of serum MDA, liver TBARS, hepatic cholesterol and triglyceride. Hamsters fed with 10% fermentation product of A. blazei (GF3), which used black bean as a part of fermentation medium, had lower levels of hepatic cholesterol and serum MDA. Both fermentation products of A. blazei could raise the content of α-tocopherol in serum. It is evident from this study that the fermentation products of A. blazei show in vivo antioxidative activity and have the potential antiatherogenic ability.
Subjects
豆科植物
巴西洋菇
倉鼠
leguminous plants
Agaricus blazei
hamsters
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-94-R92641010-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):373c63f5abe1b49706cc9d63e7ed4556