行政院國家科學委員會專題研究計畫成果報告:豬生殖與呼吸綜合症病毒感染對豬肺臟細胞素表現之影響
Date Issued
2000
Date
2000
Author(s)
DOI
892313B002077
Abstract
In this study, we used reverse transcriptionquantitative
competitive polymerase chain
reaction（RT-qcPCR）to assay porcine cytokines
and porcine reproductive and respiratory
syndrome virus（PRRSV）mRNA expression, and
investigated the effect of PRRSV infection on
cytokine gene expression in the lung tissue of
nursing pigs. We modified a previously
described RT-qcPCR method to construct a DNA
fragment to measure interleukin-1b（IL-1b）,
IL-2, IL-4, IL-8, interferon-g（IFN-g）, tumor
necrosis factor-a, b-actin, and PRRSV mRNA
expression in pig. This constructed DNA
fragment had identical primer sequences to target
cDNA except differences in the size of products.
After co-amplifying of RNA-derived cDNA with
serial dilution of the DNA fragment and
calculating the ratios between them, a precise
quantification data of sample was obtained. By
using this DNA fragment, it was possible to
analyze IL-1b, IFN-g and PRRSV mRNA
expression in the lung between PRRSV-infected
（n=4）and sham-inoculated（n=4）piglets. The
results revealed that piglets infected by PRRSV
had a higher level of IFN-g mRNA expression in
their lung than those of sham-inoculation（ P＜
0.05）at 7, 14, and 21 days post-inoculation（PI）.
PRRSV- infected piglets also showed higher IL-
1b mRNA expression than sham-inoculated ones
at 21 days PI（ P＜0.05）although there were no
differences between the groups of 7 and 21 days
PI. Besides, as virus load increased IFN-g and
IL-1b mRNA expression would also elevated.
From these results, it is concluded that PRRSV
infection would alter IFN-g and IL-1b mRNA
levels in the lung tissue of infected piglets;
however the disturbance of immune system in
piglet by PRRSV infection required further
study.
Subjects
Porcine reproductive and respiratory
syndrome (PRRS)
syndrome (PRRS)
reverse transcriptionquantitative
polymerase chain reaction (RTqcPCR)
polymerase chain reaction (RTqcPCR)
cytokines
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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