The Design of Narrow Band Infrared Emitter and Its Applications in Cancer Treatments
Date Issued
2014
Date
2014
Author(s)
Tsai, Shang-Ru
Abstract
Recent studies found that infrared radiation (IR) can regulate physiology of organisms, stimulate mitochondrial electron transport chain, and affect expression of the genes, thereby regulating the morphology of cell growth. The goal of this thesis is to design the cell incubator equipped with IR emitter, and investigate the effectiveness and mechanisms of different wavelengths of IR on the cancer therapy.
In the first topic of this thesis, it was clarified that the effect of mid-infrared radiation (MIR) with the peak wavelengths of 3~5 um range on the growth of lung cancer cell A549. The tri-layers Au/SiO2/Au waveguide thermal emitter (WTE) was used as the light source of narrow band IR. IR was originated from the resonance of cavity mode generated in the SiO2 layer between two Au films. The results showed that IR can inhibit the growth of cancer cell A549, leading to the collapse of mitochondrial membrane potential.
In the second topic of this thesis, it was investigated that the effects of HeLa cells treated with Paclitaxel combined with 3~5 um MIR. Narrow band IR from WTE can promote the effectiveness of Paclitaxel through the collapse of mitochondrial membrane potential.
In the third topic of this thesis, it was investigated that the effects of combination treatment of 810 nm near infrared radiation (NIR) laser and photodynamic therapy (PDT) in osteosarcoma cancer cell MG-63. The data showed that 810 nm NIR laser can regulate the electron transport chain and generate a large amount of ATP to enhance the uptake of lysosome-localized photosensitizer mono-L-aspartyl chlorin (e6) (NPe6). Therefore, overproduction of reactive oxygen species was activated through red light-induced PDT effects.
The results of this thesis imply that the combination of cancer therapy and IR-regulated physiological activity can enhance the therapeutic effects on cancer cells. In future, IR can be applied to clinical and home health care to achieve therapeutic purposes without damaging normal tissue based on the characteristics of non-invasive and low side effects.
Subjects
光動力療法
低能量光療法
波導熱輻射紅外光發射器
HeLa 子宮頸癌細胞
A549 肺癌細胞
MG-63骨肉癌細胞
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-103-F95921105-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):af1966c7ac0df33ac7c9c59194e78a7c