Ultraviolet-absorbance measurement using a fluorescent transduction mechanism
Journal
Spectrochimica Acta Part A: Molecular Spectroscopy
Journal Volume
50
Journal Issue
3
Pages
577-582
Date Issued
1994
Author(s)
Abstract
A novel method for determining absorbance is described that is especially applicable to remote environmental measurements. Fluorescent dyes (Stilbene 420 and 3-hydroxyflavone) placed before and after the sample provide an internally-referenced signal proportional to transmission that is independent of the source light intensity. Concentrations as low as 50 ppb (w/w) of biphenyl in ethanol were detectable using this technique. This detection limit is comparable to that of standard laboratory absorption spectrometer measurements. © 1994.
Type
journal article