Comparative Studies on the Gene Expression of Regulatory T Cells by DNA Microarray Analysis
Date Issued
2008
Date
2008
Author(s)
Chiang, Wen-Jan
Abstract
It is widely known that a population of T cells named “regulatory T cells” (Tregs) might control the immune response in humans and rodents, maintain the self-tolerance and immune homeostasis. For the past few years, except for the surface marker CD25, immunologists found Foxp3 to be the key master factor in naturally occurring CD4+CD25+ T cells. However, there still have many questions about development, differentiation and mechanisms in the subset of Tregs even including their interaction between each other that need to be answered. herefore, in our study, we exploited lentivirus system carrying IL-10 gene to infect T cells. Making T cells over-express IL-10 gene and converted it into an IL-10-secreting Tr1-like T cell. Following that, we used the DNA microarray technique for gene analysis and compared with the data of Foxp3- and TGF-β-transduced T cells in our laboratory, then selected some target genes for examination of gene expression both in fresh and activated Treg compared with non-Treg. In addition, we isolated naïve T cells (CD4+CD25-) from BALB/c mice and treated in the presence of TGF-β in vitro and the cells can be induced to express Foxp3, converted it into peripheral inducible Treg. We also investigated gene expression of the target genes in these TGF-β-derived Foxp3+ T cells.n our basic finding, there were 37 genes expression in both of the microarray data, and we narrowed down to 11 genes which we interest and further investigate, such as Gsk3b, Ogt, Dub1, Ptprj, Ccr2, Lck, Icos, Ptprk, Slc2a1, Adm and Crem. Notably, we also found that the two genes were highly expressed in the data of Foxp3- and TGF-β-transduced T cells, and have been considered as specific markers of natural Treg in several reports, such as Gzmb and Nkg7. However, there is still needed to further investigate the relationship between these genes and nTreg.
Subjects
Regulatory T cell (Treg)
Naturally occurring CD4+CD25+Foxp3+ T cell (nTreg)
Inducible regulatory T cells (iTreg)
Type 1 regulatory T cell (Tr1)
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