Effect of cell membrane structure of human erythrocyte on its electrophoresis
Resource
Colloids and Surfaces B: Biointerfaces 32 (3): 203-212
Journal
Colloids and Surfaces B: Biointerfaces
Journal Volume
32
Journal Issue
3
Pages
203-212
Date Issued
2003
Date
2003
Author(s)
Abstract
The electrophoretic behavior of human erythrocyte is investigated theoretically. The present analysis extends previous studies to that the effect of the membrane structure of a cell is taken into account. The result obtained provides a useful tool for the estimation of the surface properties of human erythrocyte through electrophoretic measurements. Fitting the present model to the experimental data of Haydon and Seaman [Arch. Biochem. Biophys. 122 (1967) 126], for example, reveals that the surface charge of erythrocyte arises mainly from the dissociation of three functional groups, which bond to different sites on cell membrane: (a) N-acetylneuraminic acid (sialic acid) with pK HANaa=2.6, (b) some kind of α-carboxylic acid with pK HAα-ca=3.35, (c) weak amino base functional group with pK BH+=8.5. The distribution of Na+ in the protein layer of a cell is found to vary with the pH of the bulk liquid phase, the possible implication of this phenomenon to cell physiology is discussed. © 2003 Elsevier B.V. All rights reserved.
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Type
journal article
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