Studies on the release of okadaic acid in Prorocentrum lima and the methodological optimization of okadaic acid purification

This research utilize the release of okadaic acid in Prorocentrum lima to probe the best purification method of okadaic acid. When growing to the steady phase in experiment, cells begin to release okadaic acid to media fast which makes better purificaiton than directly from cell. This research utilize SP825 resin absorb okadaic acid in media, and then purify it. In the experiment of okadaic acid content analysis among different Prorocentrum lima , the differences of okadaic acid content are among Prorocentrum lima strains, and cell, and media. The strains #1a, #2 and #15 have relatively high okadaic acid content per cell. And SP825 resin adsorbability of okadaic acid is 4.736 mg / g at least, however SP825 resin deadsorbent rate can be up to 96.9%. To extract okadaic acid using dichloromethane partition SP825 methanol eluent, before running silica gel flash column. The mobile phase are hexane and isopropanol. After combining toxin dichloromethane fraction, it’s reverse phase high performance liquid chromatography purification and then we can get 95% pure okadaic acid. From deadsorption okadaic acid with SP825 to purification, it’s certainly easier procedures and less time and money consuming. .
In the experiment of P. lima culture with SP825 teabag, the result shows the okadaic acid SP825 teabag group producing is far less than control group. The author infers that okadaic acid is a key factor as environmental pressure of P. lima. When okadaic acid in media increases, it results in P. lima releasing more okadaic acid instinctively. Besides, in the experiment of okadaic acid content analysis among different Prorocentrum lima , the differences of okadaic acid content are among Prorocentrum lima strains, and cell, and media. The strains #1a, #2 and #15 have relatively high okadaic acid content per cell suiting okadaic acid purification.