Copper sulfide nanoassemblies for catalytic and photoresponsive eradication of bacteria from infected wounds
Journal
ACS Applied Materials and Interfaces
Journal Volume
13
Journal Issue
7
Pages
7865-7878
Date Issued
2021
Author(s)
Abstract
Bovine serum albumin (BSA)-encapsulated copper sulfide nanocrystals (CuS NCs) were prepared by heating an alkaline solution containing copper ions and BSA without an additional sulfur source. At a high BSA concentration (0.8 mM), nanoassembly of the as-formed CuS NCs occurs to form BSA?CuS NCs as a result of the formation of BSA gel-like structures. In addition to their intrinsic photothermal properties, the BSA?CuS NCs possess rich surface vacancies and thus exhibit enzyme-like and photodynamic activities. Spontaneous generation of hydrogen peroxide (H2O2) led to the in situ formation of copper peroxide (CPO) nanodots on the BSA?CuS NCs to catalyze singlet oxygen radical generation. The antimicrobial response was enhanced by >60-fold upon NIR laser irradiation, which was ascribed to the combined effect of the photodynamic and photothermal inactivation of bacteria. Furthermore, BSA?CuS NCs were transdermally administered onto a methicillin-resistant Staphylococcus aureus-infected wound and eradicated >99% of bacteria in just 1 min under NIR illumination due to the additional peroxidase-like activity of BSA?CuS NCs, transforming H2O2 at the infection site into hydroxyl radicals and thus increasing the synergistic effect from photodynamic and photothermal treatment. The BSA?CuS NCs exhibited insignificant in vitro cytotoxicity and hemolysis and thus can serve as highly biocompatible bactericides in preclinical applications to effectively eradicate bacteria. ? 2021 American Chemical Society.
Subjects
Bacteria; Biocompatibility; Copper oxides; Hydrogen peroxide; Infrared devices; Mammals; Metal ions; Oxidation; Peroxides; Sulfur compounds; Alkaline solutions; Bovine serum albumins; Methicillin-resistant staphylococcus aureus; NIR laser irradiation; Peroxidase-like activities; Photodynamic activities; Photothermal properties; Spontaneous generation; Sulfide minerals; antiinfective agent; bovine serum albumin; copper; nanoparticle; sulfide; catalysis; chemistry; drug effect; laser; particle size; photochemistry; photochemotherapy; Staphylococcus aureus; surface property; wound infection; Anti-Bacterial Agents; Catalysis; Copper; Lasers; Nanoparticles; Particle Size; Photochemical Processes; Photochemotherapy; Serum Albumin, Bovine; Staphylococcus aureus; Sulfides; Surface Properties; Wound Infection
Type
journal article