Development of a Microchip to Separate Micro/Nano Bio-particles by Dielectrophoresis
Date Issued
2007
Date
2007
Author(s)
Hsu, Jen-Hao
DOI
zh-TW
Abstract
本研究利用介電泳原理搭配微機電製程方式，成功製作出測試人體血液中分離出γ球蛋白的分離晶片，整個流程僅需兩道光罩（微電極陣列晶片與微流道母模各一）即可完成晶片製作。本實驗採用的γ球蛋白與 Anti-Human IgG皆由瑞博公司所購買，再與購買自MERCK公司的粒子，粒子上面有一層羧基群(carboxyl group)，使Anti-Human IgG鍵結聚苯乙烯粒子表面，再讓Anti-Human IgG與γ球蛋白中的IgG作反應，成為一複合體粒子。吾人將紅血球與此複合體粒子作混合，在吾人所製作的生物晶片中，成功做出分離現象，操作電壓為10Vpp、頻率2MHz。由於此生物晶片可以於低電壓下迅速分離紅血球與人體血清蛋白質，所以此微分離晶片之開發對於人體上的應用，將有極大的幫助。此晶片在生物實驗方面，將可以幫助在使用免疫擔體沉澱法時，只需應用上少量的抗體與抗原，減少醫療耗費即可做出蛋白質的分析。
This study presents a new bio-chip which can separate the gamma globulin from the human serum based on dielectrophoresis forces. MEMS fabrication process was used to produce the bio-chip and only two photomasks were required in the experiment. Anti-human IgG was bound on the polymer beads surface with carboxyl group to be complex beads. Gamma globulin was then mixed with the complex beads heterogeneously. The mixtures which contained both bio-particles and RBCs were injected into the bio-chip to test the separation efficiency. The driving source, 10Vpp and 2MHz, was used to separate RBCs and bio-particles. The current of this chip was less than human perception current so that it was safe for biologic tests. Furthermore, Immunoprecipitation showed that small amount of samples were needed for this chip to detect the antibody or antigen easily.
Subjects
介電泳
γ球蛋白
Anti-Human IgG
Dielectrophoresis
Gamma globulin
Type
thesis