High-throughput quantification of osteogenesis in WJ-MSCs using Alizarin Red S, von Kossa, and Picro-Sirius red.
Journal
Future science OA
Journal Volume
11
Journal Issue
1
Start Page
Article number 2567169
ISSN
2056-5623
Date Issued
2025-12
Author(s)
Huang, Chung-You
Abstract
Background: Osteogenic differentiation refers to the process by which mesenchymal stem cells (MSCs) transform into bone tissues. The microplate reader, characterized by its requirement for fewer samples, simple operation, excellent repeatability, and rapid detection speed, has been utilized to measure the absorbance of cells undergoing osteogenic differentiation. Research design and methods: In this study, we employed the microplate reader to analyze the histological stains on Wharton’s Jelly Mesenchymal Stem Cells (WJMSCs) in vitro, aiming to streamline future osteogenic differentiation studies. Results: It was not recommended to use a microplate reader to read Optical Density (OD) values for Alizarin red S and Picro-Sirius red (PSR) staining. However, a microplate reader could be effectively used to read OD values at wavelengths of 596, 620, and 680 nm from day 3 to day 5 in von Kossa staining (VKS) results. Conclusions: Thus, while the microplate reader is recommended for early-stage VKS quantification, its use is not advised for Alizarin red S staining (ARS) or PSR assays.
Subjects
Alizarin red S
Picro-Sirius red
Wharton’s Jelly mesenchymal stem cells
microplate reader
optical density values
osteogenic differentiation
von Kossa staining
SDGs
Type
journal article