Immunomagnetic Reduction for Fetal Fibronectin Detection - a Novel Method to Detect the Preterm Biomarker
Resource
BIOMEDICAL ENGINEERING-APPLICATIONS BASIS COMMUNICATIONS v.23 n.4 pp.273 -278
Journal
Biomedical Engineering: Applications, Basis and Communications
Journal Volume
v.23
Journal Issue
n.4
Pages
27-3
Date Issued
2011
Date
2011
Author(s)
Abstract
Preterm birth is the main cause of perinatal morbidity and mortality throughout the world. Fetal fibronectin (fFN) is one type of glycoprotein detected in the interface of the choriodecidual junction. Preterm labor is related to the disruption of the choriodecidual junction and thus fFN releases in the ectocervix or posterior vaginal fornix. Quantization of fFN can assist the diagnosis and prevention of preterm birth. In this work , we developed an immunoassay, the immunomagnetic reduction (IMR), to quantitatively detect fFN. It was found that the low detection limit for fFN via IMR is less than 10 ng/ml, which is much lower than clinic criteria 50 ng/ml. In addition to the high sensitivity, IMR assay shows such merits as low cost and high reliability for detecting fFN.
Subjects
Preterm birth
Fetal fibronectin
Immunomagnetic reduction