Specific Gene Expression of Nitroreductase in Zebrafish Primordial Germ Cells for the Inhibition of Gonadal Development
Date Issued
2009
Date
2009
Author(s)
Lin, Pei-Yu
Abstract
In the past decades, the transgenic fish of various species are being actively investigated worldwide for increasing efficiency of aquaculture production. However, the commercialization of genetically modified fish is restricted beside of transgenic ornamental fish. The global concern about ecological issue is genetically modified (GM) fish which may cause gene-flow and the threat of extinction of natural stock. In view of this issue, sterility control technology becomes important technology to solve this problem. Traditional sterility techniques such as polyploidy technology and knockdown of reproductive hormone by antisense RNA are not totally reliable. Furthermore, even through these sterile course are totally sterility, the fish stock can’t be inherited for maintenance; hence inducible sterile technology must be needed to develop for maintaining the fish stock. Nitroreductase (NTR), which is an enzyme with the ability to converse metronidazole substrate into cytotoxin and leading to cell death. In the present study, a 3 kb nanos promoter and a 5 kb askopos (kop) promoter were cloned and used to trigger NTR expression specifically in primordial germ cells (PGCs) which are germ cells give rise to gametes involved in reproduction. Through the consecutive immersion of 5 mM Mtz at early stage and juvenile gonad stage, the reproductive capacity of transgenic fish is reduced comparing to those without Mtz treatment. Our results also demonstrated that apoptosis of juvenile ovary mediated by NTR/Mtz system causes degeneration of juvenile ovary and lead to development of defective testis structure in all male fish. These results can provide as a model for inducible control the sterility of GM fish and we expected this model can offer economic benefits for maintenance of superior breeds in aquaculture purpose.
Subjects
transgenic fish
sterility control
primordial germ cells
nitroreductase
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