Effectiveness of bacillus licheniformis-fermented products and their derived antimicrobial lipopeptides in controlling coccidiosis in broilers
Journal
Animals
Journal Volume
11
Journal Issue
12
Date Issued
2021
Author(s)
Abstract
This study aimed to investigate the potential of Bacillus licheniformis-fermented products (BLFP) and their derived antimicrobial lipopeptide, surfactin, for the prevention of coccidiosis in broilers. Broilers were fed BLFP at 1.25 and 5 g/kg under Eimeria tenella challenge. At the end of experiment (35 days), the growth performance, survival rate, cecal morphology, cecal lesion scores, oocyst-count index, and anti-coccidial index were analyzed. The effects of the BLFP-derived surfactin on oocyst sporulation and sporozoite morphology in Eimeria species were also investigated in vitro. Results showed that BLFP supplementation at 1.25 and 5 g/kg improved cecal morphology and increased the survival rate of broilers under E. tenella challenge. Supplementation with 1.25 g/kg of BLFP reduced the lesion scores in the cecum of E. tenella-challenged broilers, while the oocyst-count index was reduced in broilers given 5 g/kg of BLFP. The anti-coccidial index of the 1.25 g/kg of BLFP-treated group was greater than 160, compared with the E. tenella-challenge-only group. Furthermore, surfactin inhibited Eimeria oocyst sporulation and disrupted sporozoite morphology. These results demonstrate that BLFPs and their derived antimicrobial lipopeptide, surfactin, exhibit anti-coccidial activity in vitro and in vivo. BLFP may be used as a natural feed additive for the prevention of coccidiosis in broilers, and 1.25 g/kg can be considered the optimum dosage. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Antimicrobial lipopeptide; Bacillus licheniformis; Broiler; Coccidiosis; Surfactin
SDGs
Other Subjects
alcohol; alpha tocopherol; coccidiostatic agent; folic acid; food additive; lipopeptide; pyridoxine; selenium; silicon dioxide; surfactin; taurodeoxycholic acid; trace element; trypsin; animal experiment; animal model; antimicrobial activity; Article; Bacillus licheniformis; bacterium contamination; body weight; coccidiosis; diet supplementation; DNA extraction; fermented product; food intake; intestine flora; microscopy; nonhuman; oocyst; sporocyst; sporogenesis; survival rate
Type
journal article