CIP2A is a predictor of poor prognosis in colon cancer
Journal
Journal of Gastrointestinal Surgery
Journal Volume
16
Journal Issue
5
Pages
1037-1047
Date Issued
2012
Author(s)
Kuen-Feng Chen
Abstract
Purpose The cancerous inhibitor of protein phosphatase 2A (CIP2A) oncoprotein is overexpressed in colon cancer tissue compared to normal colon mucosa. We investigated the impact of CIP2A on colon cancer. Methods A tissue microarray consisting of 167 colon cancer specimens was investigated. The association between CIP2A and clinicopathological parameters was analyzed using the χ2 test. Survival was analyzed using the Kaplan-Meier method. The impact of CIP2A on proliferation and drug resistance was evaluated using the 3-(4, 5-dimethylthiazolyl-2)-2, 5- diphenyltetrazolium bromide test. An anchorage-independent colony formation assay was also performed. Results CIP2A was an independent prognostic factor in colon cancer after controlling for other clinical confounding factors, such as stage and lymphovascular invasion, particularly in stages III and IV (hazard ratio02.974, P<0.001). The knockdown of CIP2A reduced the proliferation and anchorage-independent colony formation of colon cancer cells. Knockdown of CIP2A decreased the resistance of the cells to 5-fluorouracil, oxaliplatin, and SN38 (an active metabolite of irinotecan). Treatment with 5-fluorouracil, oxaliplatin, and SN38 decreased CIP2A expression. Conclusions CIP2A is a prognostic factor in colon cancer. The knockdown of CIP2A reduced proliferation and anchorageindependent colony formation and increased 5-fluorouracil, oxaliplatin, and SN38 efficacy in colon cancer cell lines. ? 2012 The Society for Surgery of the Alimentary Tract.
Subjects
Chemotherapy; Cip2a; Colon cancer; Mapk pathway; Proliferation
SDGs
Other Subjects
antineoplastic agent; autoantigen; KIAA1524 protein, human; membrane protein; RNA; tumor marker; adenocarcinoma; adult; aged; article; cell proliferation; colon tumor; colorectal tumor; comparative study; drug effect; female; genetics; human; immunohistochemistry; Kaplan Meier method; male; metabolism; methodology; middle aged; mortality; needle biopsy; pathology; predictive value; prognosis; protein microarray; real time polymerase chain reaction; risk assessment; survival; tumor cell culture; Western blotting; Adenocarcinoma; Adult; Aged; Aged, 80 and over; Antineoplastic Agents; Autoantigens; Biopsy, Needle; Blotting, Western; Cell Proliferation; Colonic Neoplasms; Colorectal Neoplasms; Female; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Male; Membrane Proteins; Middle Aged; Predictive Value of Tests; Prognosis; Protein Array Analysis; Real-Time Polymerase Chain Reaction; Risk Assessment; RNA, Neoplasm; Survival Analysis; Tumor Cells, Cultured; Tumor Markers, Biological
Type
journal article