Using the aminomethyl-chemmatrix beads to achieve the purpose of protein equalization
Date Issued
2009
Date
2009
Author(s)
Huang, Tz-Yun
Abstract
Most of biological extracts (e.g., serum, pleural effusion) have an extremely wide dynamic concentration range of over ten orders of magnitude, so a few well-characterized, high-abundant proteins mask the signal of other low abundance proteins. The separation and detection of low abundant proteins in the proteome is still an obstacle to be solved. Many methods which can make the “hidden proteome” become easier to research are developed in recently. We report here the use of amiomethyl-chemmatrix beads to reduce the dynamic concentration range in pleural effusion (PE). The amiomethyl-chemmatrix beads have the amphiphilic nature to equalize the concentration of different protein species. After treatment with the bead, the low abundance proteins in PE are easier to detect by 2-DE PAGE. The differences in the treated PE from normal person and lung cancer person are easier determined by 2-DE PAGE and we expect that the cancer biomarkers will be discovered among the different proteomes between these two treated PE.
Subjects
aminomethyl-chemmatrix bead
protein equalization
SDGs
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