Study of Linker & Interfacial Effect on the Quartz Crystal Microbalance in Aqueous Condition

Traditional prediction about frequency reduction of Quartz Crystal Microbalance (QCM) after materials bonding was challenged by the phenomenon of warfarin binding and denature by urea. The response was classified as the interfacial effect after eliminating the mass and the viscoelasticity effects. Multi-parameter detection that includes the mass, the viscoelastic effect, etc. was detected and analyzed by using high-overtone Quartz Crystal Microbalance (HQCM) in this thesis. Furthermore, QCM was for the first time to make the observation of the pure transition of hydrophilic to hydrophobic property possible during the case of urea unfolding.
HQCM was fabricated and integrated into a complete system that enables multiple parameters measurement. The general biological interaction inducing mass, viscoelastic, interfacial effect will be more apparently described and analyzed on the platform of HQCM. At the same time, the lab-built system provides much freedom, having potential to develop advanced study of interface science. The whole system can potentially find application in drug discovery.