Effect of Kefir on Glucose Uptake in Skeletal Muscle
Date Issued
2015
Date
2015
Author(s)
Wu, Chen-Pei
Abstract
Diabetes mellitus (DM) is a predominant chronic disease which causes mortality of millions of people yearly. Insulin resistance is a key etiology of Type 2 DM. Kefir is a fermented milk which composed a specific mixture of bacteria and yeast cultures, and have received increasing attention as potential ingredients of health-promoting functional foods. Past studies revealed that kefir can improve diet-related chronic diseases, such as obesity, systemic inflammation, hyperglycemia, hypercholesterolemia and type 2 DM, but the exact mechanism is still unknown. In our study, a free fatty acid induced insulin resistant cell model was first established. We found that when pH is higher than 4.5, the lactic acid bacteria and the yeast in kefir could help each other grow up steadily. With longer fermentation time the glucose uptake activity of L6 cell became higher and along with dose response. According to results of ultrafiltration (1 kDa), it indicated that the active compound might be small molecular protein and peptide. The water extract compound from 12 hours and 96 hours fermentation under 37℃ not only activated AMPK signaling molecules, but also PI3K signaling molecules to induce GLUT4 translocation. The water extract compound from 12 hours and 24 hours fermentation of traditional kefir and 24 hours fermentation under 37℃ primary activated PI3K signaling molecules, the signaling molecules pathway is similar with insulin. The water extract compound from 96 hours fermentation of traditional kefir primary activated AMPK signaling molecules. Furthermore, the water crude extracts was fractionated by anion-exchange chromatography. It was found that the fraction 5 of co-culture kefir at 37℃ has high glucose uptake activity, and it both activated AMPK and PI3K signaling molecules to induce GLUT4 translocation.
Subjects
Type 2 diabetes mellitus
insulin resistance
glucose uptake
skeletal muscle
kefir
co-culture
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-104-R02641007-1.pdf
Size
23.54 KB
Format
Adobe PDF
Checksum
(MD5):ba464d6a4bdec536fe0e5ccc76ecac41