Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators
Resource
Phytochemistry, 69(6), 1348-1358
Journal
Phytochemistry
Journal Volume
69
Journal Issue
6
Pages
1348-1358
Date Issued
2008
Author(s)
Shyur, Lie-Fen
Huang, Chi-Chang
Lo, Chiu-Ping
Chiu, Chih-Yang
Chen, Yi-Ping
Wang, Sheng-Yang
Abstract
Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl4-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC50 = 5 μg/mL) and TPA-induced COX-2 protein expression in mouse skin (1 mg/200 μL/site). CJH7-2 (10 mg/kg BW) can prevent CCl4-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection. ? 2008 Elsevier Ltd. All rights reserved.
Subjects
Cryptomeria japonica; Cycloxygenase-2; Heme oxygenase-1; Hepatoprotection; Inducible nitric oxide synthase; Nitric oxide; Taxodiaceae; Terpenoids
SDGs
Other Subjects
carbon tetrachloride; prostaglandin synthase; prostaglandin synthase inhibitor; terpene; animal; article; cell line; chemical structure; chemically induced disorder; chemistry; drug effect; female; inflammation; Institute for Cancer Research mouse; Japanese cedar; liver disease; macrophage; male; metabolism; mouse; skin; wood; Animals; Carbon Tetrachloride; Cell Line; Cryptomeria; Cyclooxygenase Inhibitors; Female; Inflammation; Liver Diseases; Macrophages; Male; Mice; Mice, Inbred ICR; Molecular Structure; Prostaglandin-Endoperoxide Synthases; Skin; Terpenes; Wood; Coniferophyta; Cryptomeria japonica; Cupressaceae; Mus
Type
journal article
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