Surface plasmon resonance biochips for tuberculosis bacillus detection
Journal
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Journal Volume
26 IV
Pages
2553-2556
Date Issued
2004
Author(s)
Abstract
Tuberculosis is one of notifiable infectious diseases which may cause serious epidemic problems. Traditional diagnostic techniques include acid- fast stain, bacteria culture, metabolic monitoring have some disadvantages such as low sensitivity, time consumption and less specificity. In this study we proposed a new diagnostic technique based on the protein chips concept which detected by SPR phenomena. It was found that the resonance angle (?51.86°) shifted slightly toward right with antibody concentration of 10X, 30X, 100X, and 300X dilution under optimal concentration of immobilized TB antigen W38 (MW 41.5 kDa, 50 μg/ml). While for the W06 (MW 14 kDa, 50 μg/ml), the resonance angle was around 50.13° and shifted with the same trend as W38 did.
SDGs
Other Subjects
Antibodies; Bacteria; Concentration (process); Diseases; Molecular biology; Monolayers; Photoresists; Proteins; Biochips; Glass substartes; Protein chips; Tuberculosis; Surface plasmon resonance
Type
conference paper
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