Quantitative susceptibility mapping-based microscopy of magnetic resonance venography (QSM-mMRV) for in vivo morphologically and functionally assessing cerebromicrovasculature in rat stroke model
Journal
PLoS ONE
Journal Volume
11
Journal Issue
3
Pages
1 - 22
Date Issued
2016
Author(s)
Abstract
Abnormal cerebral oxygenation and vessel structure is a crucial feature of stroke. An imaging method with structural and functional information is necessary for diagnosis of stroke. This study applies QSM-mMRV (quantitative susceptibility mapping-based microscopic magnetic resonance venography) for noninvasively detecting small cerebral venous vessels in rat stroke model. First, susceptibility mapping is optimized and calculated from magnetic resonance (MR) phase images of a rat brain. Subsequently, QSM-mMRV is used to simultaneously provide information on microvascular architecture and venous oxygen saturation (SvO2), both of which can be used to evaluate the physiological and functional characteristics of microvascular changes for longitudinally monitoring and therapeutically evaluating a disease model. Morphologically, the quantification of vessel sizes using QSM-mMRV was 30% smaller than that of susceptibility-weighted imaging (SWI), which eliminated the overestimation of conventional SWI. Functionally, QSM-mMRV estimated an average SvO2 ranging from 73% to 85% for healthy rats. Finally, we also applied QSM to monitor the revascularization of post-stroke vessels from 3 to 10 days after reperfusion. QSM estimations of SvO2 were comparable to those calculated using the pulse oximeter standard metric. We conclude that QSM-mMRV is useful for longitudinally monitoring blood oxygen and might become clinically useful for assessing cerebrovascular diseases. © 2016 Hsieh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
SDGs
Other Subjects
analytical error; animal experiment; animal model; Article; biological monitoring; blood vessel reactivity; brain blood flow; brain vein; cerebral revascularization; cerebrovascular accident; controlled study; disease model; functional assessment; in vivo study; male; microvasculature; morphology; nonhuman; nuclear magnetic resonance imaging; nuclear magnetic resonance scanner; phlebography; physiological process; pulse oximeter; quantitative susceptibility mapping based microscopic magnetic resonance venography; rat; reperfusion; susceptibility weighted imaging; vein diameter; venous oxygen tension; animal; blood; brain circulation; diagnostic imaging; magnetic resonance angiography; microcirculation; oximetry; pathophysiology; phlebography; procedures; Sprague Dawley rat; oxygen; Animals; Cerebrovascular Circulation; Disease Models, Animal; Magnetic Resonance Angiography; Male; Microcirculation; Oximetry; Oxygen; Phlebography; Rats; Rats, Sprague-Dawley; Stroke
Type
journal article