S100a8/a9 enhances immunomodulatory and tissue-repairing properties of human amniotic mesenchymal stem cells in myocardial ischemia-reperfusion injury
Journal
International Journal of Molecular Sciences
Journal Volume
22
Journal Issue
20
Date Issued
2021
Author(s)
Abstract
Paracrine factors of human mesenchymal stem cells (hMSCs) have the potential of prevent-ing adverse cardiac remodeling after myocardial infarction (MI). S100A8 and S100A9 are calcium-binding proteins playing essential roles in the regulation of inflammation and fibrous tissue formation, and they might modulate the paracrine effect of hMSCs. We isolated human amniotic mesenchy-mal stem cells (hAMSCs) and examined the changes in the expression level of regulatory genes of inflammation and fibrosis after hAMSCs were treated with S100A8/A9. The anti-inflammatory and anti-fibrotic effects of hAMSCs pretreated with S100A8/A9 were shown to be superior to those of hAMSCs without S100A8/A9 pretreatment in the cardiomyocyte hypoxia/reoxygenation experiment. We established a murine myocardial ischemia/reperfusion model to compare the therapeutic effects of the conditioned medium of hAMSCs with or without S100A8/A9 pretreatment. We found the hearts administered with a conditioned medium of hAMSCs with S100A8/A9 pretreatment had better left ventricular systolic function on day 7, 14, and 28 after MI. These results suggest S100A8/A9 enhances the paracrine therapeutic effects of hAMSCs in aspects of anti-inflammation, anti-fibrosis, and cardiac function preservation after MI. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Cardiac remodeling
Human amniotic mesenchymal stem cells
Myocardial infarction
Paracrine effects
S100A8
S100A9
calcium binding protein
calgranulin A
calgranulin B
S100A8 protein, human
S100A9 protein, human
animal
C57BL mouse
cardiac muscle cell
cell culture
disease model
drug effect
fibrosis
gene expression regulation
heart infarction
human
immunomodulation
inflammation
ischemia
male
mesenchymal stem cell
metabolism
mouse
myocardial ischemia reperfusion injury
pharmacology
physiology
Animals
Calcium-Binding Proteins
Calgranulin A
Calgranulin B
Cells, Cultured
Disease Models, Animal
Fibrosis
Gene Expression Regulation
Humans
Immunomodulating Agents
Immunomodulation
Inflammation
Ischemia
Male
Mesenchymal Stem Cells
Mice
Mice, Inbred C57BL
Myocardial Infarction
Myocardial Reperfusion Injury
Myocytes, Cardiac
SDGs
Type
journal article