Functional analysis of a lectin receptor kinase in BABA-mediated priming
Date Issued
2008
Date
2008
Author(s)
Lin, Grace Jui-Chih
Abstract
“Priming” is defined as a physiological condition in which plants are able to better or more rapidly mount defense responses to biotic or abiotic stresses. Many natural or synthetic compounds can alert plants to enter a so-called ‘alarmed’ or ‘primed state.’ However, the molecular mechanism of priming remains unclear at this moment. To elucidate its mechanism, the priming inducer, β-aminobutyric acid (BABA), was employed as a tool to discover genes involved in priming. The xenobiotic BABA enhances plant resistance to a wide range of biotic and abiotic stresses without a significant tradeoff in plant fitness. Twenty eight Arabidopsis T-DNA insertion lines selected from a group of BABA-responsive genes were tested for their responses to BABA- inhibition of root growth and BABA-induced resistance (BABA-IR) to the virulent bacteria, Pseudomonas syringae pv tomato DC3000 (Pst DC3000). A lectin receptor kinase mutant (lecRK-a1) demonstrated enhanced BABA-mediated root growth inhibition and a deficiency in BABA-IR against Pst DC3000. Absence of PR-1 (PATHOGENESIS-RELATED 1) potentiation was correlated with this deficiency even though the lecRK-a1 mutant still responded normally towards other virulent and avirulent pathogens. We conclude that this lectin receptor kinase is necessary for BABA-IR and BABA-mediated priming of Arabidopsis defense responses towards virulent bacteria. Since LecRK-a1 is induced early during infection, it may be involved in plant-microbe recognition.
Subjects
priming
Arabidopsis thaliana
β-aminobutyric acid
lectin
receptor protein kinase
stress
pathogen response
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