Interpretation on Band-broadening in Chromatography with Spatial Peak Profiles Obtained Using Whole-column Detection
Resource
Journal of the Chinese Chemical Society 56: 480-484
Journal
Journal of the Chinese Chemical Society
Journal Volume
56
Journal Issue
3
Pages
480-484
Date Issued
2009
Date
2009
Author(s)
Abstract
Abstract Fifteen liquid chromatographic experiments were investigated using a whole‐column detection (WCD) system and a conventional post‐column UV/Vis detector. The peak widths obtained from chromatograms were found dependent on the retention factor; the larger the retention factor was the greater the peak width. However, the on‐column spatial peak widths were dependent on the locations where they were measured in the column. The peak widths monitored at 17 cm from the column inlet were found essentially the same no matter what their retention factors were. In addition, a linear relationship was found between the chromatographic peak width and the reciprocal of the average linear rate of the solute migration. The peak widths on chromatograms did not reflect how they appeared in the column; instead, the widths were determined by the solute speed passing the detector.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
33.pdf
Size
931.4 KB
Format
Adobe PDF
Checksum
(MD5):e430a01295266db683e74985ed5e0612