The Preparation of Magnetic Bamboo Charcoal as a Template for High-efficiency Cytoflow-bioreactor Base Cell Sorter(HCBCS): Monoclonal Antibody Production
Date Issued
2011
Date
2011
Author(s)
Chen, Hao-Lin
Abstract
George Kohler and Cesar Milstein employed fusion theory to produce monoclonal antibody in 1975. Monoclonal antibodies (mAbs) have been proven useful in research and clinical applications. However, the generation of mAbs by conventional hybridoma technology is time-, cost- and labor-consuming. Before, a siphon bioreactor and the carrier(bamboo charcoal) combined as a cell sorter called : Cytoflow-bioreactor base cell sorter(CBCS). Our design allows the production of mAbs while avoiding time-consuming steps, such as large numbers of limiting dilutions and screening assays, and demonstrates that the CBCS could be a powerful tool for monoclonal antibody production. But the CBCS still had some problems : the particle size of carrier is limited、non-specific binding、low-efficiency cell sorting and operational difficulties.
The purpose of study is improving cytoflow-bioreactor base cell sorter, promote cell sorting efficiency, avoid the possibility of non-specific binding and operational difficulties, create a high-efficiency cytoflow-bioreactor base cell sorter(HCBCS) could be employed to harvest antibody-secreting B cells from the peripheral blood without sacrificing the animal.
In the future, we hope this method not only produce animals monoclonal antibody but also produce humanized monoclonal antibody, to achieve the purpose of monoclonal antibody mass production.
Subjects
Bioreactor
Hybridomas
Cytoflow reactor
Monoclonal antibody
Magnetic Bamboo charcoal
Surface modification
Type
thesis
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