S100P immunostaining identifies a subset of peripheral-type intrahepatic cholangiocarcinomas with morphological and molecular features similar to those of perihilar and extrahepatic cholangiocarcinomas
Journal
Histopathology
Journal Volume
61
Journal Issue
6
Pages
1106-1116
Date Issued
2012
Author(s)
Abstract
Aims: S100P is a calcium-binding protein that is frequently expressed in pancreatic adenocarcinoma and perihilar cholangiocarcinoma. The aim of this study was to investigate the pathological significance of the expression of S100P in peripheral intrahepatic cholangiocarcinoma (ICC). Methods and results: Immunohistochemical staining was used to investigate S100P expression in 112 cases of peripheral ICC. The results were compared with those for perihilar and extrahepatic cholangiocarcinomas. Patients with S100P-positive peripheral ICC were more likely to have elevated serum levels of carcinoembryonic antigen (CEA) and CA19-9 than those with S100P-negative peripheral ICCs. All cases of peripheral ICC associated with intrahepatic lithiasis and all cases with intraductal/periductal growth patterns were positive for S100P. S100P-positive peripheral ICCs were highly associated with 'bile duct' morphology rather than cholangiolar differentiation. Nearly all cases of perihilar and extrahepatic cholangiocarcinoma were positive for S100P. Similarly to perihilar and extrahepatic cholangiocarcinomas, S100P-positive peripheral ICCs showed more frequent expression of CEA and MUC2, and were more likely to be N-cadherin-negative, than S100P-negative cases. Notably, K-RAS mutations were only detected in S100P-positive peripheral ICCs, with a frequency similar to that in perihilar and extrahepatic cholangiocarcinomas. Patients with S100P-positive peripheral ICC were more likely to have poor prognoses than those with S100P-negative tumours. Conclusions: S100P immunostaining identifies a subset of peripheral ICC that probably originates from larger bile ducts. This subset of peripheral ICCs shares common morphological and molecular features with perihilar and extrahepatic cholangiocarcinomas. ? 2012 Blackwell Publishing Limited.
Other Subjects
CA 19-9 antigen; calcium binding protein; carcinoembryonic antigen; K ras protein; mucin 2; nerve cell adhesion molecule; S100P protein; unclassified drug; adult; aged; article; bile duct; bile duct cancer; bile duct carcinoma; biliary tract surgery; blood sampling; cancer morphology; cancer patient; clinical feature; controlled study; diagnostic test accuracy study; extrahepatic cholangiocarcinoma; female; gene frequency; gene mutation; histopathology; human; human cell; human tissue; immunohistochemistry; major clinical study; male; perihilar cholangiocarcinoma; peripheral intrahepatic cholangiocarcinoma; predictive value; priority journal; protein blood level; protein expression; sensitivity and specificity; serum; tissue section; tumor differentiation; Adult; Aged; Aged, 80 and over; Antigens, Tumor-Associated, Carbohydrate; Bile Duct Neoplasms; Bile Ducts, Extrahepatic; Bile Ducts, Intrahepatic; Calcium-Binding Proteins; Carcinoembryonic Antigen; Cholangiocarcinoma; Diagnosis, Differential; Female; Humans; Kaplan-Meier Estimate; Male; Middle Aged; Neoplasm Proteins; Prognosis; Retrospective Studies; Tumor Markers, Biological
Type
journal article