Proteomics Analysis of SlSOBIR1-interacting Tobacco Proteins Involved in Elicitin Signaling
Date Issued
2014
Date
2014
Author(s)
Huang, Chun-Tzu
Abstract
Recognition of microbe/pathogen-associated molecular patterns (MAMPs/PAMPs) by plasma membrane-localized pattern recognition receptors (PRRs) is the initial step to activate PAMP-triggered immunity (PTI) in plants. In addition to PRRs, other leucine-rich repeat receptor-like kinases are known to play important roles in PTI, including BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1 (BAK1), BAK1-INTERACTING RECEPTOR-LIKE KINASE1 (BIR1), and SUPPRESSOR OF BIR1-1/EVERSHED (SOBIR1/EVR), which interact with the receptors to regulate downstream defense responses. Recently, tomato homologs of SOBIR1 (known as SlSOBIR1 and SlSOBIR1-like) are known to mediate plant basal defense against Phytophthora parasitica. Furthermore, they are involved in the signaling of ParA1, an elicitin of P. parasitica. In this study, coimmunoprecipitation (co-IP) and mass spectrometry were performed to identify SlSOBIR1-interacting proteins to find out how SlSOBIR1 mediates ParA1 signaling in Nicotiana benthamiana. A total of 157 SlSOBIR1-interacting proteins were identified, of which 25 of them are associated with SlSOBIR1 only in the presence of ParA1. GO term analysis indicated that three biological process terms were specifically enriched in response to ParA1 treatment, including cell communication, response to endogenous stimulus, and response to stress. To uncover their roles, we silenced 11 of the 25 genes by TRV-mediated gene silencing, and then expressed ParA1 by agroinfiltration. ParA1-induced necrosis is compromised upon silencing of NbArcA2 and NbGBLP (guanosine nucleotide-binding proteins, G protein), NbPDR1 [ATP-binding cassette tranporter (ABC) transporter], NbPR10 [pathogenesis-related (PR) protein], NbOligoA (Oligopeptidase A), and NbrbohB (NADPH oxidase), with silencing of NbPDR1 showing the most prominent effect.
Subjects
PAMP-triggered immunity
receptor-like kinase
共免疫沉澱
交互作用蛋白體
elicitin
疫病菌
Type
thesis
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