The association analysis of levels of beta-arrestin 2 in peripheral blood mononuclear cells and clinopathological manifestations of asthma in Taiwan children.
Date Issued
2014
Date
2014
Author(s)
Kao, Yu-Hsuan
Abstract
Introduction
Asthma has been rapidly increasing in prevalence over decades in children as well as in adults. Beta-arrestins, originally discovered as molecules that bind to and desensitize the activated and phosphorylated form of the G protein-coupled beta2-adrenergic receptor (β2-AR), have recently emerged as multifunctional adaptor/scaffold proteins that dynamically assemble a wide range of multiprotein complexes. Furthermore, beta-arrestin 2 knockout animals had been found failure to develop the signs of allergic asthma (airway hyperreactivity). We conducted this study to survey the association between the levels of beta-arrestin 2 in peripheral blood mononuclear cells and clinopathological manifestations of asthma in Taiwanese children.
Methods
This study included children with (1) asthma or allergic rhinitis, (2) atopic dermatitis but without asthma or allergic rhinitis, and (3) normal control. They all received blood sampling for detecting β-arrestin 2 in PBMCs (peripheral blood mononuclear cells) by WB (Western blots) or FACS (Fluorescence Activated Cell Sorting). The asthma severity, eosinophil count, total IgE, specific IgE, cytokines, and lung function were also recorded.
Results
95 Taiwan children are evaluated including 11 healthy children, 83 patients with asthma and only 1 child with atopic dermatitis. In the WB groups, the mean level of β-arrestin 2 in the asthmatic children was higher than the level of β-arrestin 2 in the only one child with atopic dermatitis. In our FACS results, the b-arrestin 2 amounts were higher in children with mild persistent asthma than mild intermittent asthma.(p=0.054) The expression level of β-arrestin 2 has a significantly positive correlation with total IgE (r=0.254, p=0.028).
Conclusions
Our study was the first report about that β-arrestin 2 may be involved in asthma by studying PBMCs in human. The serum levels of total IgE was a predictive factor of the expression of β-arrestin 2. The levels of eosinophil counts had interaction effect between the levels of b-arrestin 2 and total IgE.
Subjects
氣喘
孩童
乙型制動素-2
總免疫球蛋白E
西方點墨法
螢光激活細胞分選術
SDGs
Type
thesis
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