Screening and Characterization of AtGSTU17-Interacting Proteins in Arabidopsis thaliana
Date Issued
2011
Date
2011
Author(s)
Chen, Shiang-Jiun
Abstract
Glutathione S-transferase (GST) is an enzyme which catalyses the conjugation of reduced glutathione to electrophilic centers on a wide variety of substrates. This reaction is involved in detoxification of a wide range of compounds. The plant GSTs have been assigned to six classes, and tau class (GSTUs) is the largest one. In addition to playing role in oxidative stress response, some GSTs have been found to participate in regulatory function in plant growth and development. AtGSTU17 has been found involved in photonorphogenesis and in abiotic stress response. In order to understand how AtGSTU17 participates in the abiotic stress response, we use yeast two-hybrid to screen for the protein has physical interaction with AtGSTU17. Fifteen proteins are identified in the test and play function in development, metabolism, transcription regulation, abiotic and biotic stress response. We choose ATHM2, one of the AtGSTU17-interacting proteins for further studies. ATHM2 belongs to thioredoxin M class protein and is predicted to involve in osmotic stress response (TAIR web). The AtGSTU17-ATHM2 interaction is confirmed by using yeast two-hybrid and biomolecular fluorescence complementation (BiFC). When AtGSTU17 and ATHM2 are individually expressed, AtGSTU17 accumulates in cytoplasm and ATHM2 localize in chloroplasts. However, the subcellular localization of AtGSTU17-ATHM2 protein complex is localized in chloroplasts. We suggest the transport of AtGSTU17 from cytoplasm to chloroplast is mediated by the signal peptide at ATHM2. To futher understand the relationship between AtGSTU17 and ATHM2, we are studying two homozygous T-DNA insertion lines from SALK Institude, Salk_130686C and Salk_123570C. Comparing the phenotypes of athm2s with wild type seedlings, the germination percentages of athm2 mutants are lower and the root length is shorter. However, no difference of the oxidative stress tolerance was found between wild type and athm2 mutants.
Subjects
Arabidopsis thaliana
oxidative stress
glutathione S-transferase
thioredoxin
Type
thesis
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