血紅生成素與缺氧相關因子在急性與慢性水腦症之基因表現

Hydrocephalus is the most common disease in pediatric neurosurgery. It is caused
by increased production of cerebrospinal fluid (CSF), blockage of CSF pathway, or
impaired absorption of CSF. The abnormal accumulation of CSF in the brain during
hydrocephalus will have effects to the brain not only from mechanical compression
and metabolic disturbance, but also from vascular compromise, which will impair the
cerebral oxygen supply.
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It is known that hypoxia induces transcription of erythropoietin (EPO) and other
hypoxia-inducible genes, such as vascular endothelial growth factor (VEGF). It is
hypothesized that the increased intracranial pressure (IICP) during hydrocephalus
changes the expression of EPO and other hypoxia-inducible genes. Using RT-PCR
and Western blot analysis, the aims of this study are as follows:
1. The expression of EPO and other hypoxia-inducible genes in the brain tissue
during hydrocephalus
2. The location of EPO and other hypoxia-inducible genes in the brain tissue
during hydrocephalus (Is it different with the results of other studies in the
hypoxic brain)
3. The relationship between the intracranial pressure and the expression of EPO
and other hypoxia-inducible genes
4. The chronological changes of expression of EPO and other hypoxia-inducible
genes in different stages of hydrocephalus
5. The treatment effect (CSF diversion) to the expression of EPO and other
hypoxia-inducible genes