TRIM5alpha restrains Epstein-Barr virus lytic cycle by mediating ubiquitination of Rta
Date Issued
2011
Date
2011
Author(s)
Chen, Chien-Sin
Abstract
An immediate-early protein, Rta, is an important transcription factor required for initiating the lytic cycle of Epstein-Barr virus (EBV). Tripartite-motif 5 alpha (TRIM5alpha) is a restriction factor of retroviruses with the ubiquitin E3 ligase activity, which protects primates form retrovirus infection in a species-specific manner. However, the documented target spectrum of TRIM5alpha was limited to RNA viruses. This study finds that TRIM5alpha is a binding partner of Rta and colocalizes with Rta in the cytoplasm. The interaction involves the N-terminal region in Rta and the C-terminal B30.2 domain in TRIM5alpha. Moreover, overexpression of TRIM5alpha facilitates the ubiquitination of Rta and reduces the level of Rta, thereby inhibiting the transactivation activity of Rta. Collectively, this study identifies Rta to be the first DNA viral target of human TRIM5alpha, and the interaction prevents EBV lytic progression by promoting Rta''s ubiquitination.
Subjects
ubiquitin
ubiquitination
lytic cycle
Type
thesis
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