Using a Fourier Hyperspectral microscopy image-based system to quantify the optical parameters of oral mucosa
Date Issued
2011
Date
2011
Author(s)
Li, Yi-Shan
Abstract
In this thesis, we used a Fourier transfer hyperspectral image-based microscopy spectrometer combined with a imaging fiber-optic bundle to measure reflectance spectrum at different source-detector separation. We also used a fitting tool to extract the optical parameters and quantify the hemoglobin concentration and oxygen saturation.
We used scaling Monte carlo as the forward model to construct a two-layer oral mucosa spatially-resolved reflectance spectrum. In inversed model, we used Levenberg-Marquart algorism to fitting reflectance spectrum, in order to extract the optical parameters and the thickness of epithelium. We used a oral simulated oral spatially-resolved reflectance spectrum with random noise to validate fitting tool. The result indicate that combined with multi-fiber reflectance spectrum, we can extract the absorption and reduced scattering coefficients of oral double-layer tissue simultaneously and get the thickness of the epithelium. The root mean square error of optical parameters are all below 10%.
We used phantom to validate the study method. We choose polystyrene sphere as scatter, red ink as absorber and water as medium to simulate a single-layer tissue phantom, and used a standard phantom to calibration reflectance spectrum, and get spatially-resolved reflectance spectrum successfully by our optical system. The mean percent error of our measuring method to extract the optical parameters of homogeneous phantom is 0.72%、-1.6%、3.5% for polystyrene concentration, polystyrene diameter and red ink concentration, respectively. The root mean square error of absorption coefficients and reduced scattering coefficients is 4.33% and 2.5%. For two-layer tissue validation, we used TiO2 as scatter and red ink as absorber to fabricate two-layer phantom, and used PDMS and agar as medium to compare the measurement results and to find the best phantom fabrication and measurement protocol. The extracted optical parameters are all below 15%.
We used Fourier transfer hyperspectral image-based microscopy spectrometer to measure the reflectance spectrum of oral mucosa, and used a standard phantom to calibration the system response. Successfully get the reflectance of oral mucosa at wavelength range of 450 nm to 800 nm and using the optical parameter extracting tool to get the normal human oral mucosa optical parameters.
Subjects
Oral mucosa
spatially-resolved reflectance spectrum
optical parameter
tissue phantom
Fourier transfer hyperspectral image-based microscopy spectrometer
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-100-R98945004-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):53288bc0c71a56d53ca6abf200e9c0ed