The E3 ubiquitin ligase SIAH2 is a prosurvival factor overexpressed in oral cancer
Journal
Anticancer Research
Journal Volume
33
Journal Issue
11
Pages
4965-4974
Date Issued
2013
Author(s)
Abstract
Background: This study aimed at investigating the expression and functional significance of seven in absentia homologues 2 (SIAH2), an E3 ubiquitin ligase, in oral squamous cell carcinoma (OSCC). Materials and Methods: Comparative genomic hybridization and real-time PCR were performed to examine the amplification of SIAH2 gene in clinical specimens of OSCC tissues. Expression of SIAH2 mRNA and protein was examined by quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assays, respectively. Apoptosis was examined by annexing V staining and Poly (ADP-ribose) polymerase (PARP) cleavage, with or without siennamediated SIAH2 knockdown. The expression of p53 was analyzed by immunoblotting. Results: Levels of SIAH2 DNA and mRNA were significantly greater in clinical OSCC specimens and in cultured OSCC cells, which also stained positively for the SAIH2 protein. Knockdown of SIAH2 led to growth suppression and apoptosis induction in a p53- independent mechanism. Conclusion: These results revealed a tight correlation of SIAH2 overexpression with OSCC and suggest an oncogenic role of SIAH2 in oral cancer.
Subjects
Apoptosis; Oral cancer; Siah2; Ubiquitin ligase
SDGs
Other Subjects
protein p53; significance of seven in absentia homolog 2; ubiquitin protein ligase E3; unclassified drug; adult; apoptosis; article; cancer cell; cancer inhibition; cancer survival; clinical article; comparative genomic hybridization; female; human; immunoblotting; immunohistochemistry; male; middle aged; mouth squamous cell carcinoma; priority journal; protein analysis; protein expression; real time polymerase chain reaction; reverse transcription polymerase chain reaction; apoptosis; Oral cancer; Siah2; ubiquitin ligase; Adult; Aged; Apoptosis; Blotting, Western; Carcinoma, Squamous Cell; Chromosome Aberrations; Comparative Genomic Hybridization; Female; Follow-Up Studies; Gene Expression Regulation, Neoplastic; Humans; Immunoenzyme Techniques; Male; Middle Aged; Mouth Neoplasms; Neoplasm Grading; Neoplasm Staging; Nuclear Proteins; Prognosis; Real-Time Polymerase Chain Reaction; Retrospective Studies; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA, Small Interfering; Tumor Cells, Cultured; Tumor Markers, Biological; Ubiquitin-Protein Ligases
Type
journal article