Toxicogenomics of A375 human malignant melanoma cells treated with arbutin
Resource
Journal of Biomedical Science 14 (1): 87-105
Journal
Journal of Biomedical Science
Pages
87-105
Date Issued
2007
Date
2007
Author(s)
Cheng, Sun-Long
Liu, Rosa Huang
Sheu, Jin-Nan
Chen, Shui-Tein
Sinchaikul, Supachok
Tsay, Gregory Jiazer
Abstract
Although arbutin is a natural product and widely used as an ingredient in skin care products, its effect on the gene expression level of human skin with malignant melanoma cells is rarely reported. We aim to investigate the genotoxic effect of arbutin on the differential gene expression profiling in A375 human malignant melanoma cells through its effect on tumorigenesis and related side-effect. The DNA microarray analysis provided the differential gene expression pattern of arbutin-treated A375 cells with the significant changes of 324 differentially expressed genes, containing 88 up-regulated genes and 236 down-regulated genes. The gene ontology of differentially expressed genes was classified as belonging to cellular component, molecular function and biological process. In addition, four down-regulated genes of AKT1, CLECSF7, FGFR3, and LRP6 served as candidate genes and correlated to suppress the biological processes in the cell cycle of cancer progression and in the downstream signaling pathways of malignancy of melanocytic tumorigenesis. ? 2006 National Science Council.
Subjects
A375 cells; Arbutin; DNA microarray; Gene expression; Toxicogenomics
SDGs
Other Subjects
arbutin; article; cancer growth; carcinogenesis; cell composition; cell cycle; controlled study; correlation analysis; DNA microarray; down regulation; gene control; gene expression profiling; gene repression; genotoxicity; human; human cell; melanocyte; melanoma cell; nucleotide sequence; priority journal; toxicogenetics; unindexed sequence; Arbutin; Cell Line, Tumor; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Melanoma; Oligonucleotide Array Sequence Analysis; Skin Neoplasms; Toxicogenetics
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