Steroids from the soft coral Sinularia crassa
Journal
Marine Drugs
Journal Volume
10
Journal Issue
2
Pages
439-450
Date Issued
2012
Author(s)
Abstract
One new sterol, crassarosterol A (1), and four new steroidal glycosides, crassarosterosides A-D (2-5) were isolated from the Formosan soft coral Sinularia crassa. The absolute configuration of 1 was determined using the Mosher's method. The absolute configurations for the sugar moieties of 2-5 were determined by HPLC analysis on the o-tolylthiocarbamates derived from the liberated sugar after acid hydrolysis. Compounds 2 and 4 could significantly inhibit the expression of pro-inflammatory iNOS protein at 10 ?M. In contrast, 1-3 were found to stimulate the expression of COX-2 protein at this concentration. Steroids 1 and 4 also showed cytotoxicity toward the selected human liver cancer cells. ? 2012 by the authors; licensee MDPI.
Subjects
Anti-inflammatory activity; Crassarosterol A; Crassarosterosides A-D; O-tolylthiocarbamate; Sinularia crassa
SDGs
Other Subjects
crassarosterol A; crassarosteroside A; crassarosteroside B; crassarosteroside C; crassarosteroside D; cyclooxygenase 2; inducible nitric oxide synthase; natural product; steroid; unclassified drug; antiinflammatory activity; antineoplastic activity; article; cancer cell; concentration response; controlled study; coral; drug cytotoxicity; drug determination; drug hydrolysis; drug isolation; drug structure; high performance liquid chromatography; human; human cell; liver cancer; protein expression; Sinularia crassa; structure analysis; Alcyonacea; Sinularia
Type
journal article