Studies of Migration Behavior and Enantioseparation of Antihistamines in Capillary Zone Electrophoresis
Date Issued
2004
Date
2004
Author(s)
Huang, Wen-Ping
DOI
zh-TW
Abstract
Capillary zone electrophoresis has proven to be a powerful technique for enantioseparation. In this study, five chiral antihistamines were investigated using cyclodextrins as chiral selectors. The results of this thesis are presented in two parts:
In the first part, we focus on the binding constants of antihistamines with addition of neutral cyclodextrins (HP-β-CD, β-CD, DM-β-CD). In this work, the interaction of chiral analytes with chiral selectors was studied through the variation of mobility as a function of neutral cyclodextrins concentration. Binding constants of antihistamines to HP-β-CD, β-CD and DM-β-CD were evaluated through curve-fitting. The results indicate that the interaction of antihistamines with neutral cyclodextrins, which are also affected by antihistamines structures, play a
major role in the determination of enantioseparation.
In the second part , we focus on the enantioseparation of antihistamines with addition of randomly sulfate-substituted β-CD (MI-S-β-CD) and single-isomer heptakis (2,3-dihydroxy-6-O-sulfo )-β-cyclodextrin (SI-S-β-CD) and the optimization of separation. Moreover, by mixing MI-S-β-CD with SI-S-β-CD, the enantioseparation efficiency is considerably enhanced. In comparison with the use of SI-S-β-CD alone, the enantiomers of three antihistamines could be baseline separated when mixed cyclodextrins were used.
Subjects
毛細管區帶電泳法
對掌異構物
抗組織胺
環糊精
Capillary Zone Electrophoresis
Antihistamines
cyclodextrins
Enantioseparation
Type
thesis
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