Triflavin, an Arg-Gly-Asp-Containing Antiplatelet Peptide Inhibits Cell- Substratum Adhesion and Melanoma Cell-Induced Lung Colonization
Resource
JAPANESE JOURNAL OF CANCER RESEARCH v.83 n.8 pp.885-893
Journal
JAPANESE JOURNAL OF CANCER RESEARCH
Journal Volume
v.83
Journal Issue
n.8
Pages
885-893
Date Issued
1992
Date
1992
Author(s)
Abstract
Triflavin, an Arg-Gly-Asp (RGD) containing peptide purified from Trimeresurus flavoviridis snake venom, inhibits human platelet aggregation by blocking fibrinogen binding to fibrinogen receptors associated with glycoprotein IIb/IIIa complex. In this study, we show that triflavin (1-30 micrograms/mouse) inhibits B16-F10 melanoma cell-induced lung colonization in C57BL/6 mice in a dose-dependent manner . In vitro, triflavin dose-dependently inhibits adhesion of B16-F10 melanoma cells to extracellular matrices (ECMs; i.e. , fibronectin, fibrinogen, vitronectin, and collagen type I) . Triflavin is approximately 600-800 times more potent than GRGDS at inhibiting cell adhesion. In addition, triflavin dose -dependently inhibits B16-F10 cell-induced platelet aggregation. These results imply that the inhibitory effect of triflavin on the adhesion of tumor cells to ECMs (e.g., fibronectin, vitronectin and collagen type I) and/or tumor cell-induced platelet aggregation may be partially responsible for its antimetastatic activity in C57BL/6 mice.
Subjects
RGD-CONTAINING PEPTIDE,TUMOR CELL METASTASIS,EXTRACELLULAR
MATRIX