Using Bio-organic field effect transistor for bio-sensing
Date Issued
2009
Date
2009
Author(s)
Tsai, Yi-Jiun
Abstract
In recent year, the requirements of the personal healthcare are rsing significantly.Beacuse of the urgent requirements on the market, the researches of low-cost disaposable bio-sensors are one of the major topic in the filed. Researchers suggest many new types of bio-snesor to people, and among of the all, the field-effect-transistor(NWFET、CNT FET) gets the lots of attention because of its high sensitivity and ease of use properties. However, higher cost limited the widely commercial use of theses types of sensor. The most popular type of bio-sensor in personal healthcare is electrochemical biosensors because of its stablity and relatively lower cost. If we can combine the advantages of two types of biosensors as a new type biosensor, the new type of sensor will be most competitive and has potential in commercial market. From this viewpoint, we proposed a new type of biosensor mechanism which combines the structures of the two types of biosensors and will be verified in this thesis. We use the bio-material (AFP and S100) and the high conductive linker (CT5) to be the semiconductor layer of our bio-organic field effect transitor. We found the structure constructed by two materials can truly have field effect. When applying gate voltage, Vg, -20V to the chip, we can get the current difference enlarged for two times at most, compared with pure electrochemical effect without the external electric field built-up by the applied gate voltage. In the concentration tests on S100 protein on the bio-organic field effect transitor with -20V Vg,the sensitivity of the sensor could rise from non-detectable to 1μg/mL. In the end, we use the BSA molecule to verify the specific binding effect. The measured current signal became much smaller than in the specific binding situation so as we can claim the strucute of this sensor can truly detect the specific binding and the sensitivity of the chip is achieve 1μg/mL.
Subjects
Bio-sensing:field-effect transistor
Type
thesis
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