Effective components of Nelumbo nucifera Gaertn. seed embryo on anti-aging of Caenorhabditis elegans
Date Issued
2011
Date
2011
Author(s)
Wu, Meng-Ying
Abstract
Aging is accumulation of diverse deleterious changes in the cells and tissues advancing with age that increase the risk of disease and death. Aging changes can be attributed to development and genetic defects, the environment, disease process, and an inherent process, referred to as the aging process. According to the world population composition, the proportion of older people and the aging index continue to rise. It is assumed that the proportion of elderly people (>60 years old) will rise to 21% of the world population. The associated problem will also rise. Therefore, anti-aging will be an important issue in the society.
There are a lot of factors possess the anti-aging activity. One of these factors is food intake. Our group has evaluated the anti-aging activity of many extracts from the flower, fruit and herbal. One of these is Nelumbo nucifera Gaertn. seed embryo. Caenorhabditis elegans is an animal model which has short life cycle and its genome is well defined. Besides, it is easily to raise under the lab environment. Therefore, the C. elegans is chosen as the model to screen out the anti-aging compound(s) in the N. nucifera Gaertn. seed embryo. We have found that the fifth fraction of n-butanol fraction of methanol extract of N. nucifera Gaertn. seed embryo (Nn-M-B-V) can ignificantly extend the mean lifespan of C. elegans by 8.5%. Therefore, HPLC was employed to separate Nn-M-B-V and two sub-fractions (Nn-M-B-V-3 and Nn-M-B-V-4) were collected. According to the result of lifespan test, we found that the Nn-M-B-V-3 can significantly extend the mean lifespan by maximum 11.86% and minimum 3.54%.
In the past research, we assumed that the Nn-M-B-V-3 is flavonoid. We try to separate and purify the Nn-M-B-V-3. The LC/MSn result suggested that Nn-M-B-V-3 contain 3 major compounds, which has the same molecule weight—564. That, these 3 major compounds were separated by HPLC and collected for NMR analysis. According to the NMR result, we preliminary assume that peak-2 is a diglycoside flavonoid.
Subjects
Caenorhabditis elegans
aging
Nelumbo nucifera Gaertn. seed embryo
nature product purification
Type
thesis
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