Cytogenetic studies on human liver cancer cell lines
Journal
Cancer Genetics and Cytogenetics
Journal Volume
65
Journal Issue
2
Pages
161-166
Date Issued
1993
Author(s)
Abstract
Karyotyping of human liver cancer cell lines and chromosome in situ hybridization of hepatitis B virus (HBV) DNA was performed in order to elucidate the possible mechanism of hepatocarcinogenesis from evidence of chromosomal changes and HBV integration patterns. HepG2-2 and HepG2-5 cell lines were HepG2 cells experimentally transfected with HBV DNA; the HCC36 cell line was derived from a hepatocellular carcinoma containing integrated HBV DNA from a Taiwanese patient. HepG2 cells, a hepatoblastoma cell line without HBV DNA integration, served as negative control. In HepG2-2, HepG2-5, and HCC36 cells, multiple integrations of HBV DNA were observed by in situ hybridization and hybridization signals occurred preferentially on certain chromosomes: 2, 5, 10, 11, 18; 7, 10, 13, 17, 18; and 4, 6, 11, 12q+, 18; respectively. In addition, a strong correlation between chromosomal changes and HBV integration was noticed in HCC36 cells, especially at chromosome 12q+. ? 1993.
SDGs
Other Subjects
virus dna; article; cancer cell culture; chromosome 12q; chromosome analysis; controlled study; cytogenetics; genetic transfection; hep 2 cell; hepatitis b virus; human; human cell; in situ hybridization; karyotyping; liver cancer; liver carcinogenesis; priority journal; southern blotting; Carcinoma, Hepatocellular; Chromosome Aberrations; DNA, Viral; Hepatitis B Virus; Human; In Situ Hybridization; Karyotyping; Liver Neoplasms; Support, Non-U.S. Gov't; Tumor Cells, Cultured; Hepatitis B virus
Type
journal article