Processing-bodies in Drosophila S2 cells
Date Issued
2006
Date
2006
Author(s)
Chung, Chien-Che
DOI
en-US
Abstract
The degradation of eukaryotic mRNAs plays important roles in the modulation of gene expression, quality control of mRNA biogenesis and antiviral defenses. Two general pathways of mRNA decay have been identified in eukaryotic cells. After shortening of the poly(A) tail at the 3′ end of the mRNA, mRNA may be degraded in a 5’ to 3’ direction or in a 3’ to 5’ direction.
Recently, in yeast and human cells, many enzymes of the basic decay machinery are found in the cytoplasmic foci, called processing bodies (P bodies). Further experiments have proved that P-bodies are actual sites of mRNA decay, especially for 5’ to 3’ decay pathway. Based on the conserved mRNA decay factors and the similar mRNA decay mechanisms between species, we suggest that the processing body related structure probably also exists in Drosophila.
Here, we observed that the mRNA decay factors, such as Ccr4, dDcp1, dDcp2, Me31B, and Pacman, are concentrated in the cytoplasmic foci of Drosophila S2 cells. Whether these proteins are in a unique set of structures? We construct a series of proteins which involve in the different mRNA decay pathways to transfect the cells then co-immunostain the transfected protein and endogenous dDcp1 (as a marker of putative P bodies). It was found that proteins that involved in the 5’ to 3’ mRNA decay all localize to the same cytoplasmic foci, we called as dDcp1-containing bodies.
However, we can not exclude the possibility that these foci are sites for protein storage rather than mRNA decay. To answer this question, a series of experiments such as dsRNA silencing the mRNA decay factors, translational inhibitor treatment, and the visible mRNA decay intermediates are required. Finally, we may identify these dDcp1-containing bodies are the “processing bodies” in Drosophila cells.
Subjects
裂解體
訊息核醣核酸
果蠅
P-bodies
mRNA decay
Drosophila
Type
other
File(s)![Thumbnail Image]()
Loading...
Name
ntu-95-R93b43016-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):f297a9b8a04259b9a9b610da78e28936