Harnessing Viral Promoters for Targeted RNAi: Engineering Phloem-Specific Resistance Against Tomato Yellow Leaf Curl Thailand Virus
Journal
Synthetic Promoters
Series/Report No.
Methods in Molecular Biology
Part Of
Methods in Molecular Biology
Journal Volume
2844
Start Page
239
End Page
245
ISSN
1064-3745
1940-6029
ISBN (of the container)
9781071640623
Date Issued
2024
Author(s)
Abstract
Phloem-specific promoter efficiently triggers graft-transmissible RNA interference (gtRNAi). We leveraged a phloem-specific promoter derived from the Rice tungro bacilliform virus, optimizing the RNAi mechanism’s efficiency and specificity. Here, we detail the construction of phloem-specific promoter-based gtRNAi system and its application through grafting experiments, demonstrating its effectiveness in inducing tomato yellow leaf curl Thailand virus (TYLCHTV) resistance in non-transgenic scions. This strategy presents a practical application for protecting crops against viruses without genetically modifying the entire plant.
Subjects
Graft-transmissible RNA interference (gtRNAi)
Phloem-specific promoter
Rice tungro bacilliform virus (RTBV)
RNA interference (RNAi)
Tomato yellow leaf curl Thailand virus (TYLCHTV)
SDGs
Publisher
Springer US
Type
book part