Effects of Short- and Long-Lived ROS Generated by a Cold Atmospheric Plasma Jet on Human Xenograft A2058 Melanoma and Associated Side Effects in a Nude Mouse Model
Journal
IEEE Transactions on Radiation and Plasma Medical Sciences
Journal Volume
10
Journal Issue
4
Start Page
629
End Page
639
ISSN
2469-7311
2469-7303
Date Issued
2025-08-14
Author(s)
Abstract
This study evaluated the therapeutic efficacy of long-lived and short-lived reactive oxygen species (ROS) generated by argon cold atmospheric plasma (Ar-CAP) under water-soaking conditions in a murine model of treating cutaneous melanoma. Potential side effects associated with Ar-CAP treatment in mice were also assessed. While direct cold atmospheric plasma (CAP) treatment and indirect treatment via injection of plasma-treated solution have been reported in previous studies, the distinct modalities employed have made it challenging to delineate the specific contributions of long-lived and short-lived ROS in vivo. In this study, direct and indirect CAP treatments were applied to melanoma xenografts under comparable conditions. Both modalities effectively inhibited tumor progression, with direct treatment leading to a more pronounced tumor volume reduction in the early stages. Over a six-month observation of CAP-treated nude mice, no significant adverse effects were noted in pain response, skin appearance, blood cell counts, or histopathological evaluation, aside from slight elevations in liver enzymes (AST and ALT). These results suggest that argon CAP (Ar-CAP) treatment is both effective and well-tolerated, with short-lived ROS playing a key role in enhancing antitumor activity.
Subjects
Argon cold atmospheric plasma (Ar-CAP)
blood test
direct/indirect cold atmospheric plasma (CAP) treatment
histological examination
long-lived/short-lived reactive oxygen species (ROS)
melanoma tumor
mouse model
pain index assessment
side effects
Publisher
Institute of Electrical and Electronics Engineers (IEEE)
Type
journal article